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一种简单的分级提取方法，用于从单个样品中综合分析代谢物，脂质和蛋白质
JoVE 신문
생화학
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JoVE 신문 생화학
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
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一种简单的分级提取方法，用于从单个样品中综合分析代谢物，脂质和蛋白质

DOI:
10.3791/55802-v

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11:17 min

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June 01, 2017

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, , ,
1Max Planck Institute of Molecular Plant Physiology, Golm, Germany, 2Department of Pharmacognosy, Faculty of Pharmacy,Cairo University

Chapters

  • 00:05Title
  • 01:05Grinding and Tissue Disruption
  • 02:30Aliquoting Tissue
  • 03:18Extraction and Fractionation by Phase Separation
  • 04:56Aliquoting Polar and Hydrophobic Fractions
  • 06:24Concentrations and Storage of Fraction and Analysis of Lipids Using UPLC-MS
  • 07:48Analysis of Polar and Semi-polar Metabolites Using UPLC-MS
  • 08:51Results: Representative Chromatograms of Lipid, Semi-polar Metabolites and Proteins from Arabidopsis thaliana Leaf Extracts
  • 10:40Conclusion

Summary

자동 번역

자동 번역

提出了使用一个样品从生物组织中综合提取脂质，代谢物和蛋白质的方案。

Tags

Fractionated ExtractionMetabolitesLipidsProteinsSingle SampleMTBE ExtractionMultiomics AnalysisArabidopsisSample Preparation

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