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Métodos in vitro para comparar la unión de diana y la inducción de CDC entre anticuerpos terapéuticos: Aplicaciones en análisis de biosimilitud
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JoVE 신문 면역학 및 감염병학
In Vitro Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis
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Métodos in vitro para comparar la unión de diana y la inducción de CDC entre anticuerpos terapéuticos: Aplicaciones en análisis de biosimilitud

17,565 Views

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07:25 min

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May 04, 2017

DOI:

10.3791/55542-v

DOI:
10.3791/55542-v

•

07:25 min
May 04, 2017

•

3 Views
, , , ,
1Unit for Development and Research in Bioprocesses Unit (UDIBI), National School of Biological Sciences,National Polytechnic Institute (IPN), University of Mexico (UNAM), 2School of Chemistry,National Autonomous University of Mexico (UNAM), 3Graduate Program in Chemical Sciences,National Autonomous University of Mexico (UNAM), 4Unit for Development Research and Medical Innovation in Biotechnology (UDIMEB), National School of Biological Sciences,National Polytechnic Institute (IPN), 5Department of Immunology, National Scool of Biological Sciences,National Polytechnic Institute (IPN), 6Department of Pharmacology and Unit of Translational Biomedicine (CMN 20 de noviembre), School of Medicine,National Autonomous University of Mexico (UNAM)

Summary

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Este protocolo describe la comparación in vitro de dos características funcionales clave del rituximab: la unión al diana y la inducción de la citotoxicidad dependiente del complemento (CDC). Los métodos se emplearon para una comparación lado a lado entre el rituximab de referencia y un biosimilar de rituximab. Estos ensayos se pueden emplear durante el desarrollo biosimilar o como un control de calidad en su producción.

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Salinas-Jazmín, N., González-González, E., Vásquez-Bochm, L. X., Pérez-Tapia, S. M., Velasco-Velázquez, M. A. In Vitro Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis. J. Vis. Exp. (123), e55542, doi:10.3791/55542 (2017).

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