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时间分辨电喷雾电离氢氘交换质谱用于研究蛋白质结构与动态

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Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

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时间分辨电喷雾电离氢氘交换质谱用于研究蛋白质结构与动态

9,616 Views

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09:18 min

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April 17, 2017

DOI:

10.3791/55464-v

DOI:

10.3791/55464-v

•

09:18 min

April 17, 2017

•

7 Views

Cristina Lento¹, Shaolong Zhu¹, Kerene A. Brown¹, Ruth Knox¹, Peter Liuni¹, Derek J. Wilson^1,2,3

¹Department of Chemistry,York University, ²The Centre for Research in Mass Spectrometry,York University, ³The Centre for Research on Biomolecular Interactions,York University

Summary

构象的灵活性在蛋白质功能的关键作用。在此，我们描述了使用耦合到氢 - 氘交换用于探测驱动功能在有序和无序的蛋白质的快速结构变化的时间分辨电喷雾电离质谱法。

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