/
/
L'identification d'un épitope linéaire des cellules B monoclonales d'anticorps reconnus par le peptide à balayage assistée
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
L'identification d'un épitope linéaire des cellules B monoclonales d'anticorps reconnus par le peptide à balayage assistée
9,240 Views
•
08:09 min
•
March 24, 2017
DOI:
Summary
Automatically generated
Here, the authors present a simple and efficient protocol to define a linear antigenic epitope using a purified monoclonal antibody and peptide scanning through dot-blot hybridization. The identified epitope can then be used in therapeutic and diagnostic applications.
Read Article
Cite this Article
Chen, C., Chang, C. Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope. J. Vis. Exp. (121), e55417, doi:10.3791/55417 (2017).
Copy