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Developmental Biology

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JoVE Journal Developmental Biology
Isolating and Analyzing Cells of the Pancreas Mesenchyme by Flow Cytometry
 

Isolating and Analyzing Cells of the Pancreas Mesenchyme by Flow Cytometry

Article DOI: 10.3791/55344-v • 05:38 min • January 28th, 2017
January 28th, 2017 •
1, 1, 1
1Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University

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요약
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Here, we describe a method for the isolation of cells in the pancreas microenvironment from embryonic, neonatal and adult mouse tissue, focusing on the isolation of mesenchymal cells. This method allows profiling of cell gene expression and protein secretion in order to elucidate the extrinsic signals that regulate pancreas development, function, and tumorigenesis.

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Isolate Cells Pancreatic Mesenchyme Flow Cytometry Gene Expression Analysis Surface Marker Expression Analysis Pancreas Development Mesenchymal Cells Epithelial Development Advantages Of Technique Culture Dish HBSS Stereo Microscope Fine Forceps Digestive Buffer Heating Block Incubation Centrifugation Cell Resuspension
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