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Human Lung Dendritic Cells: Spatial Distribution and Phenotypic Identification in Endobronchial Biopsies Using Immunohistochemistry and Flow Cytometry
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Chapters
- 00:05Title
- 01:01Glycol Methylacrylate (GMA) Resin Endobronchial Biopsy Embedment
- 02:32GMA-embedded Endobronchial Biopsy Immunohistochemical Staining
- 05:23Endobronchial Biopsy Enzymatic Digestion and Flow Cytometric Analysis
- 08:17Results: Identifying Immune and Dendritic Cell Subsets in Endobronchial Biopsies
- 10:08Conclusion
Lung-resident immune cells, including dendritic cells (DCs) in humans, are critical for defense against inhaled pathogens and allergens. However, due to the scarcity of human lung tissue, studies are limited. This work presents protocols to process human mucosal endobronchial biopsies for studying lung DCs using immunohistochemistry and flow cytometry.
