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T淋巴细胞有丝分裂原诱导芽生的快速定量辨识免疫调节药物
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs
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T淋巴细胞有丝分裂原诱导芽生的快速定量辨识免疫调节药物

18,247 Views

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08:13 min

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December 27, 2016

DOI:

10.3791/55212-v

DOI:
10.3791/55212-v

•

08:13 min
December 27, 2016

•

5 Views
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1Department of Neuroscience, Cell Biology and Physiology, Boonshoft School of Medicine,Wright State University, 2Department of Pharmacology and Toxicology, Boonshoft School of Medicine,Wright State University

Summary

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T-lymphocyte mitogenesis is accompanied by blastogenic transformation, whereupon the cell volume enlarges before cell division. Here, we describe a method to quantify blastogenesis in T lymphocytes using an automated cell counter with the capability of measuring cell diameters.

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T LymphocyteBlastogenesisBlast TransformationImmunomodulatory DrugsAutomated Cell CounterCell DiameterT Lymphocyte ActivationT Lymphocyte ProliferationSpleenSplenocyteNylon Wool ColumnRed Blood Cell LysisRPMI Medium

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Cite this Article

Gibson, J. N., Beesetty, P., Sulentic, C., Kozak, J. A. Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs. J. Vis. Exp. (118), e55212, doi:10.3791/55212 (2016).

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