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Indlæsning af et calciumfarvestof i frøens nerveender gennem nervestumpen: Kalktransient registrering i Frog Neuromuscular Junction
JoVE 신문
신경과학
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JoVE 신문 신경과학
Loading a Calcium Dye into Frog Nerve Endings Through the Nerve Stump: Calcium Transient Registration in the Frog Neuromuscular Junction
DOI:

17:05 min

July 08, 2017

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Chapters

  • 00:00Title
  • 00:06Introduction
  • 01:03Preparing of a Dye Loading Solution
  • 01:53Dye Loading Procedure
  • 05:40Praraing the Tissue for Microscopy
  • 08:22Video Capture with Digital CCD Camera
  • 10:06Data Analysis
  • 14:13Results
  • 14:56Discussion

Summary

자동 번역

Her beskriver vi en metode til indlæsning af et kalciumfølsomt farvestof gennem frøens nervestub i nerveenderne. Vi præsenterer også en protokol til optagelse og analyse af hurtige calciumtransienter i perifere nerveender.

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