Identificación de las pequeñas proteínas de unión a la molécula-celular en un entorno nativo de Células vivas marcado por fotoafinidad

We describe here a method for identification of small molecule-binding proteins using photoaffinity labeling. The advantage of this technique is that binding and covalent labeling of the target proteins occurs within the live cellular environment, removing the risk of disrupting native protein structure and binding conditions upon cell lysis.