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A Non-invasive and Technically Non-intensive Method for Induction and Phenotyping of Experimental Bacterial Pneumonia in Mice
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Chapters
- 00:05Title
- 00:53K. pneumoniae Culture Preparation
- 02:36Murine Intratracheal (i.t.) K. pneumoniae Aspiration
- 03:26Bronchoalveolar Lavage Fluid (BALF) Collection and Analysis
- 04:23Bacterial Lung and Peripheral Tissue Load Determination
- 05:20Results: Representative Murine Systemic and Immune Cell Responses to K. pneumoniae Oropharynx Delivery
- 06:42Conclusion
Several methods have been described in the literature for modeling bacterial pneumonia in mice. Herein, we describe a non-invasive, inexpensive, rapid method for inducing pneumonia via aspiration (i.e., inhalation) of a bacterial inoculum pipetted into the oropharynx. Downstream methods for assessment of the pulmonary innate immune response are also detailed.
