/
/
Une cytométrie en flux simple méthode pour mesurer l'absorption du glucose et de glucose Expression Transporter pour monocytes dans les sous-populations de sang total
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Une cytométrie en flux simple méthode pour mesurer l'absorption du glucose et de glucose Expression Transporter pour monocytes dans les sous-populations de sang total
16,243 Views
•
06:28 min
•
August 12, 2016
DOI:
1Centre for Biomedical Research,Macfarlane Burnet Institute for Medical Research and Public Health, 2Department of Infectious Diseases,Monash University, 3Department of Microbiology and Immunology,University of Melbourne, 4Department of Microbiology,The University of the West Indies, 5Division of Experimental Medicine, Department of Medicine,University of California, San Francisco, 6Department of Medicine,Monash University
Summary
Automatically generated
Monocytes are integral components of the human innate immune system that rely on glycolytic metabolism when activated. We describe a flow cytometry protocol to measure glucose transporter expression and glucose uptake by total monocytes and monocyte subpopulations in fresh whole blood.
Read Article
Cite this Article
Palmer, C. S., Anzinger, J. J., Butterfield, T. R., McCune, J. M., Crowe, S. M. A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood. J. Vis. Exp. (114), e54255, doi:10.3791/54255 (2016).
Copy