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Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions
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Chapters
- 00:05Title
- 00:48Chinese Hamster Ovary (CHO)-Intercellular Adhesion Molecule (ICAM) Cell Plating
- 01:50T Cell Preparation
- 02:46Input/Output Hosing and Syringe Pump Preparation
- 03:59Flow Chamber Loading and Cell Stimulation
- 05:53Results: SDF-1alpha Induced T Cell Adhesion to ICAM-1 Under Shear Stress
- 06:51Conclusion
This flow adhesion assay provides a simple, high impact model of T cell-epithelial cell interactions. A syringe pump is used to generate shear stress, and confocal microscopy captures images for quantification. The goal of these studies is to effectively quantify T cell adhesion using flow conditions.
