This manuscript describes a tube formation assay to quantify the effects of a given compound or condition on angiogenesis by using endothelial cell tube formation in a controlled environment.
Xie, D., Ju, D., Speyer, C., Gorski, D., Kosir, M. A. Strategic Endothelial Cell Tube Formation Assay: Comparing Extracellular Matrix and Growth Factor Reduced Extracellular Matrix. J. Vis. Exp. (114), e54074, doi:10.3791/54074 (2016).