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構造化照明顕微鏡データの時空間画像相関分光法により定量化したT細胞における皮質アクチンの流れ
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December 17, 2015
DOI:
1Department of Physics and Randall Division of Cell and Molecular Biophysics,King’s College London, 2Academic Department of Rheumatology, Centre for Molecular and Cellular Biology of Inflammation, Division of Immunology, Infection and Inflammatory Disease,King’s College London, 3ARC Centre for Advanced Molecular Imaging, Australian Centre for NanoMedicine,University of New South Wales Australia, 4Departments of Chemistry and Physic,McGill University
Summary
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To investigate flow velocities and directionality of filamentous-actin at the T cell immunological synapse, live-cell super-resolution imaging is combined with total internal reflection fluorescence and quantified with spatio-temporal image correlation spectroscopy.
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Ashdown, G., Pandžić, E., Cope, A., Wiseman, P., Owen, D. Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data. J. Vis. Exp. (106), e53749, doi:10.3791/53749 (2015).
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