/
/
Actine corticale Débit en cellules T quantifiée par spatio-temporelle image spectroscopie par corrélation de Structured Illumination Microscopy données
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Actine corticale Débit en cellules T quantifiée par spatio-temporelle image spectroscopie par corrélation de Structured Illumination Microscopy données
9,541 Views
•
09:09 min
•
December 17, 2015
DOI:
1Department of Physics and Randall Division of Cell and Molecular Biophysics,King’s College London, 2Academic Department of Rheumatology, Centre for Molecular and Cellular Biology of Inflammation, Division of Immunology, Infection and Inflammatory Disease,King’s College London, 3ARC Centre for Advanced Molecular Imaging, Australian Centre for NanoMedicine,University of New South Wales Australia, 4Departments of Chemistry and Physic,McGill University
Summary
Automatically generated
To investigate flow velocities and directionality of filamentous-actin at the T cell immunological synapse, live-cell super-resolution imaging is combined with total internal reflection fluorescence and quantified with spatio-temporal image correlation spectroscopy.
Explore More Videos
Read Article
Cite this Article
Ashdown, G., Pandžić, E., Cope, A., Wiseman, P., Owen, D. Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data. J. Vis. Exp. (106), e53749, doi:10.3791/53749 (2015).
Copy