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Imaging Serotonergic Fibers in the Mouse Spinal Cord Using the CLARITY/CUBIC Technique
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Imaging Serotonergic Fibers in the Mouse Spinal Cord Using the CLARITY/CUBIC Technique

Imaging Serotonergic Fibers in the Mouse Spinal Cord Using the CLARITY/CUBIC Technique

DOI:
10.3791/53673-v

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09:54 min

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February 26, 2016

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, ,
1Brain Structure and Function Group,Neuroscience Research Australia, 2School of Medical Sciences,The University of New South Wales

Chapters

  • 00:05Title
  • 01:08Transparent Mouse Spinal Cord Preparation
  • 02:30Mouse Spinal Cord Clearance
  • 04:59Immunofluorescent Imaging
  • 07:18Results: Representative Serotonergic Fiber Imaging
  • 08:55Conclusion

Summary

자동 번역

자동 번역

Supraspinal projections are important for pain perception and other behaviors, and serotonergic fibers are one of these fiber systems. The present study focused on the application of the combined CLARITY/CUBIC protocol to the mouse spinal cord in order to investigate the termination of these serotonergic fibers.

Tags

CLARITY/CUBIC TechniqueSerotonergic FibersMouse Spinal CordNeuroanatomyTraditional Histology3D FeaturesIn Situ ObservationVectorsCentral Nervous SystemCardiovascular SystemTracing MethodPlastic Dissection StageFume HoodAnesthetized MouseChest OpeningHeart ExposurePeristaltic Pump TubingSaline WashBlood Perfusion

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