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Ein Semi-automatisierten Ansatz zur Vorbereitung Antikörper-Cocktails für immunphänotypische Analyse von menschlichem peripherem Blut
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
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Ein Semi-automatisierten Ansatz zur Vorbereitung Antikörper-Cocktails für immunphänotypische Analyse von menschlichem peripherem Blut

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08:17 min

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February 08, 2016

DOI:

10.3791/53485-v

DOI:
10.3791/53485-v

•

08:17 min
February 08, 2016

•

3 Views
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1Human Immune Monitoring Laboratory, Earle A. Chiles Research Institute,Providence Cancer Center, Providence Portland Medical Center, 2Sony Biotechnology, 3Beckman Coulter, Inc. Life Sciences, 4Bristol-Myers Squibb

Tags

Antibody CocktailImmunophenotypic AnalysisPeripheral BloodSemi-automated StainingReagent DispensingPipetting Accuracy2D Barcode TubesAutomated Liquid HandlerBase CocktailActivation CocktailCross-contaminationImmunomonitoring

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Koguchi, Y., Gonzalez, I. L., Meeuwsen, T. L., Miller, W. L., Haley, D. P., Tanibata-Branham, A. N., Bahjat, K. S. A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood. J. Vis. Exp. (108), e53485, doi:10.3791/53485 (2016).

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