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Mesure et analyse de la production extracellulaire acide pour Déterminer glycolytique Taux
JoVE 신문
생물학
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JoVE 신문 생물학
Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate

Mesure et analyse de la production extracellulaire acide pour Déterminer glycolytique Taux

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06:47 min

December 12, 2015

DOI:

06:47 min
December 12, 2015

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Summary

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Glycolysis is a defining metabolic marker in multiple biological systems. Monitoring glycolysis by measuring the extracellular flux of H+ is common, but requires correction to be quantitative and unambiguous. Here, we demonstrate how to gather and correct extracellular flux data to distinguish between respiratory and glycolytic sources of extracellular acidification.

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