This content is Free Access.
24,630 Views
•
06:47 min
•
December 12, 2015
DOI:
Summary
Automatically generated
Glycolysis is a defining metabolic marker in multiple biological systems. Monitoring glycolysis by measuring the extracellular flux of H+ is common, but requires correction to be quantitative and unambiguous. Here, we demonstrate how to gather and correct extracellular flux data to distinguish between respiratory and glycolytic sources of extracellular acidification.
Read Article
Cite this Article
Mookerjee, S. A., Brand, M. D. Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate. J. Vis. Exp. (106), e53464, doi:10.3791/53464 (2015).
Copy