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Isolierung der viralen Replikation Compartment angereicherte Teil nuklearen Fraktionen von Adenovirus-infizierten normalen menschlichen Zellen
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Isolierung der viralen Replikation Compartment angereicherte Teil nuklearen Fraktionen von Adenovirus-infizierten normalen menschlichen Zellen
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November 12, 2015
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We provide a novel strategy to isolate viral replication compartments (RC) from adenovirus (Ad)-infected human cells. This approach represents a cell-free system that can help to elucidate the molecular mechanisms regulating viral genome replication and expression as well as regulation of viral-host interactions established at the RC.
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Hidalgo, P., Gonzalez, R. A. Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells. J. Vis. Exp. (105), e53296, doi:10.3791/53296 (2015).
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