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הפקה של DNA פעמיים תקועים Ministrings
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February 29, 2016
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Summary
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The linear covalently closed (LCC) DNA minivector (DNA ministring) is a non-viral gene delivery vector offering high transfection efficiency and is relevant to any DNA delivery application. The production system is simple, rapid, and scalable in vivo. The following protocol provides visual step-by-step instructions for the production of DNA ministrings.
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Wong, S., Lam, P., Nafissi, N., Denniss, S., Slavcev, R. Production of Double-stranded DNA Ministrings. J. Vis. Exp. (108), e53177, doi:10.3791/53177 (2016).
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