JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:58Culturing the Cerebellar Granule Cells
- 04:47Selectively Depleting the Microglia at 6 Days In Vitro
- 06:35Results: Characterization of LME Treatment on CGCs and Neurons and Astrocytes in CGC Cultures
- 07:51Conclusion
Microglia can influence neurons and other glia in culture by various non-cell autonomous mechanisms. Here, we present a protocol to selectively deplete microglia from primary neuronal cultures. This method has the potential to elucidate the role of microglial-neuronal interactions, with implications for neurodegenerative conditions where neuroinflammation is a hallmark feature.
