Journal
/
면역학 및 감염병학
/
In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions
JoVE 신문
면역학 및 감염병학
This content is Free Access.
JoVE 신문 면역학 및 감염병학
In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions

In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions

DOI:
10.3791/52862-v

•

10:24 min

•

June 11, 2015

•

, , , , ,
1Center for Molecular and Vascular Biology, Department of Cardiovascular Sciences,KU Leuven

Chapters

  • 00:05Title
  • 01:40S. aureus Preparation for In Vitro Perfusion and In Vivo Experiments
  • 03:00In Vitro Perfusion Experiments
  • 05:53In Vivo Mesentric Perfusion Model
  • 08:08Results: von Willebrand Factor is Essential for Shear Mediated Adhesion of S. aureus to Endothelial Cells and to the Subendothelial Matrix
  • 09:38Conclusion

Summary

자동 번역

자동 번역

To study the interaction of bacteria with the blood vessels under shear stress, a flow chamber and an in vivo mesenteric intravital microscopy model are described that allow to dissect the bacterial and host factors contributing to vascular adhesion.

Tags

Bacterial AdhesionVessel WallFlow ConditionsEndovascular InfectionsInfective EndocarditisIn Vitro Flow ChamberIn Vivo Intravital MicroscopyExtracellular MatrixEndothelial CellsStaphylococcus AureusVon Willebrand FactorShear Stress

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells

Introducing Shear Stress in the Study of Bacterial Adhesion

Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung

Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization

Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions

Humanized Mouse Model to Study Bacterial Infections Targeting the Microvasculature

A Flow Adhesion Assay to Study Leucocyte Recruitment to Human Hepatic Sinusoidal Endothelium Under Conditions of Shear Stress

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions

Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli

Read Article