Journal
/
환경과학
/
Automated Gel seleção de tamanho para melhorar a qualidade das bibliotecas de sequenciação de próxima geração Preparado a partir de amostras de água do ambiente
JoVE 신문
환경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 환경과학
Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Automated Gel seleção de tamanho para melhorar a qualidade das bibliotecas de sequenciação de próxima geração Preparado a partir de amostras de água do ambiente

10,389 Views

•

13:26 min

•

April 17, 2015

DOI:

10.3791/52685-v

DOI:
10.3791/52685-v

•

13:26 min
April 17, 2015

•

3 Views
, , , , , ,
1Department of Pathology and Laboratory Medicine, Faculty of Medicine,The University of British Columbia, 2Coastal Genomics, 3British Columbia Public Health Microbiology and Reference Laboratory

Summary

Automatically generated

This manuscript describes an automated gel size selection approach for purifying DNA fragments for next-generation sequencing. The Ranger Technology provides complete automation of the entire process of agarose gel loading, electrophoretic analysis, and recovery of targeted DNA fragments allowing for high-throughput and high quality next-generation sequencing libraries.

Explore More Videos

Keyword Extraction:Next-generation SequencingEnvironmental Water SamplesDNA Library PreparationGel Size SelectionAutomated ElectrophoresisRanger TechnologyRead LengthRead Quality

Read Article

Cite this Article

Uyaguari-Diaz, M. I., Slobodan, J. R., Nesbitt, M. J., Croxen, M. A., Isaac-Renton, J., Prystajecky, N. A., Tang, P. Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples. J. Vis. Exp. (98), e52685, doi:10.3791/52685 (2015).

Download .ris file

Copy

Share Video

.

Copy