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Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
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자동 젤 사이즈 선택은 환경 물 샘플에서 준비 차세대 시퀀싱 라이브러리의 질을 향상시키기 위해

10,389 Views

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13:26 min

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April 17, 2015

DOI:

10.3791/52685-v

DOI:
10.3791/52685-v

•

13:26 min
April 17, 2015

•

3 Views
, , , , , ,
1Department of Pathology and Laboratory Medicine, Faculty of Medicine,The University of British Columbia, 2Coastal Genomics, 3British Columbia Public Health Microbiology and Reference Laboratory

Summary

Automatically generated

This manuscript describes an automated gel size selection approach for purifying DNA fragments for next-generation sequencing. The Ranger Technology provides complete automation of the entire process of agarose gel loading, electrophoretic analysis, and recovery of targeted DNA fragments allowing for high-throughput and high quality next-generation sequencing libraries.

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Keyword Extraction:Next-generation SequencingEnvironmental Water SamplesDNA Library PreparationGel Size SelectionAutomated ElectrophoresisRanger TechnologyRead LengthRead Quality

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Uyaguari-Diaz, M. I., Slobodan, J. R., Nesbitt, M. J., Croxen, M. A., Isaac-Renton, J., Prystajecky, N. A., Tang, P. Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples. J. Vis. Exp. (98), e52685, doi:10.3791/52685 (2015).

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