JoVE Journal
Immunology and Infection
Immunology and Infection
이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.
챕터
요약
This protocol describes a high-throughput qRT-PCR assay for the analysis of type I and III IFN expression signatures. The assay discriminates single base pair differences between the highly similar transcripts of these genes. Through batch assembly and robotic pipetting, the assays are consistent and reproducible.