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배아 쥐의 뇌에서 차 중뇌 도파민 신경 세포의 분리, 문화 및 장기 유지 보수
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JoVE 신문 신경과학
Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains
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배아 쥐의 뇌에서 차 중뇌 도파민 신경 세포의 분리, 문화 및 장기 유지 보수

13,664 Views

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08:45 min

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February 19, 2015

DOI:

10.3791/52475-v

DOI:
10.3791/52475-v

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08:45 min
February 19, 2015

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14 Views
, , ,
1Division of Brain Sciences, Department of Medicine,Imperial College London, 2Department of Neurosurgery,Brigham and Women’s Hospital, Harvard Medical School, 3Department of Internal Medicine, Endocrinology,Yale University School of Medicine

Summary

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The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of neurodegeneration.

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Primary Mesencephalic Dopaminergic NeuronsEmbryonic Rodent BrainsParkinson’s DiseaseNeurodegenerative DisorderCell CultureMidbrain Dopaminergic NeuronsE12.5 MouseE14.5 RatNeuronal MorphologyPhysiological Characteristics

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Weinert, M., Selvakumar, T., Tierney, T. S., Alavian, K. N. Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains. J. Vis. Exp. (96), e52475, doi:10.3791/52475 (2015).

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