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הקלטת אור עורר תגובות Postsynaptic בנוירונים מותאמים-Dark, תכשירי עכבר רשתית Slice באמצעות טכניקות הצמד התיקון
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Recording Light-evoked Postsynaptic Responses in Neurons in Dark-adapted, Mouse Retinal Slice Preparations Using Patch Clamp Techniques
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הקלטת אור עורר תגובות Postsynaptic בנוירונים מותאמים-Dark, תכשירי עכבר רשתית Slice באמצעות טכניקות הצמד התיקון

8,279 Views

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10:30 min

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February 11, 2015

DOI:

10.3791/52422-v

DOI:
10.3791/52422-v

•

10:30 min
February 11, 2015

•

6 Views
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1Anatomy and Cell Biology,Wayne State University School of Medicine, 2Ophthalmology,Wayne State University School of Medicine

Summary

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We will demonstrate how to prepare retinal slices from the mouse eye and record light responses in retinal neurons. The entire procedure is conducted in dark-adapted conditions.

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RetinaVisual SystemNeural NetworkRetinal NeuronsBipolar CellsGanglion CellsAmacrine CellsPatch ClampDark-adaptedMouse Retinal SliceLight-evoked Synaptic ResponsesVisual Signal Processing

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Cite this Article

Hellmer, C. B., Ichinose, T. Recording Light-evoked Postsynaptic Responses in Neurons in Dark-adapted, Mouse Retinal Slice Preparations Using Patch Clamp Techniques. J. Vis. Exp. (96), e52422, doi:10.3791/52422 (2015).

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