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Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations
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Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations
Chapters
- 00:05Title
- 01:40Intracellular Staining and Flow Cytometry
- 03:52K562 Killing Assay
- 06:33Results: Representative Cytotoxic and Immunosuppressive Effects of Combustible Tobacco Product Preparations
- 08:42Conclusion
Using optimized human peripheral blood mononuclear cell (PBMC) ex vivo assays, we showed that a combustible tobacco product preparation markedly suppresses receptor-mediated intracellularly secreted cytokines and cytolytic ability of effector PBMCs. These rapid assays may be useful in product evaluation and understanding the potential long-term effects of tobacco exposure.
