JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Cytométrie en flux pour les protocoles surface et intracellulaire Antigen analyses des types cellulaires de neurones
51,407 Views
•
13:05 min
•
December 18, 2014
DOI:
1Emmy Noether-Group for Stem Cell Biology, Department of Molecular Embryology, Institute of Anatomy and Cell Biology,University of Freiburg, 2Spemann Graduate School of Biology and Medicine and Faculty of Biology,University of Freiburg, 3School of Life Sciences,Keele University, 4Center for Biological Signaling Studies (BIOSS),University of Freiburg
Summary
Automatically generated
We provide a detailed description of a protocol for flow cytometric analysis of surface antigens and/or intracellular antigens in neural cell types. Critical aspects of experimental planning, step-by-step methodological procedures, and fundamental principles of flow cytometry are explained in order to enable neurobiologists to exploit this powerful technology.
Read Article
Cite this Article
Menon, V., Thomas, R., Ghale, A. R., Reinhard, C., Pruszak, J. Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types. J. Vis. Exp. (94), e52241, doi:10.3791/52241 (2014).
Copy