Two-photon intravital imaging can be used to investigate interactions among different cell types in the spinal cord in their native tissue environment in a bone marrow chimeric animal with a dorsal column traumatic spinal cord crush injury.
Evans, T. A., Barkauskas, D. S., Myers, J. T., Huang, A. Y. Intravital Imaging of Axonal Interactions with Microglia and Macrophages in a Mouse Dorsal Column Crush Injury. J. Vis. Exp. (93), e52228, doi:10.3791/52228 (2014).