This method demonstrates a technique for assessing granulocyte function by simultaneously measuring phagocytosis of bacteria and oxidative burst. Image-based flow cytometry allowed for the identification of three distinct subsets of activated granulocytes that all differed in their relative functional capacity.
McFarlin, B. K., Venable, A. S., Prado, E. A., Henning, A. L., Williams, R. R. Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro. J. Vis. Exp. (94), e52201, doi:10.3791/52201 (2014).