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Un<em> Ex Vivo</em> Laser-indotta Spinal Cord Injury modello per valutare i meccanismi di degenerazione assonale in tempo reale
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An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time
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Un<em> Ex Vivo</em> Laser-indotta Spinal Cord Injury modello per valutare i meccanismi di degenerazione assonale in tempo reale

10,741 Views

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11:18 min

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November 25, 2014

DOI:

10.3791/52173-v

DOI:
10.3791/52173-v

•

11:18 min
November 25, 2014

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2 Views
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1KY Spinal Cord Injury Research Center, Department of Neurological Surgery,University of Louisville, 2Hotchkiss Brain Institute, Department of Clinical Neurosciences,University of Calgary

Summary

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We present a protocol utilizing two-photon excitation time-lapse microscopy to simultaneously visualize the dynamics of axon and myelin injuries in real time. This proposed protocol permits studies of both intrinsic and extrinsic factors which can influence central myelinated axon fate after injury and contribute to permanent clinical disability.

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Axonal DegenerationSpinal Cord InjuryEx Vivo ModelTwo-photon MicroscopyAxonal RetractionMyelin DegenerationAxonal SwellingAxonal TransectionReal-time Imaging

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Cite this Article

Okada, S. L. M., Stivers, N. S., Stys, P. K., Stirling, D. P. An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time. J. Vis. Exp. (93), e52173, doi:10.3791/52173 (2014).

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