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对T细胞推导<em>在体外</em>从小鼠胚胎干细胞
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JoVE 신문 면역학 및 감염병학
Derivation of T Cells In Vitro from Mouse Embryonic Stem Cells
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对T细胞推导<em>在体外</em>从小鼠胚胎干细胞

10,801 Views

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08:27 min

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October 14, 2014

DOI:

10.3791/52119-v

DOI:
10.3791/52119-v

•

08:27 min
October 14, 2014

•

4 Views
, , , , ,
1Department of Biological Sciences, Hunter College and Graduate Center,City University of New York, 2Sunnybrook Research Institute, Department of Immunology,University of Toronto

Summary

Automatically generated

Mouse embryonic stem cells can be differentiated to T cells in vitro using the OP9-DL1 co-culture system. Success in this procedure requires careful attention to reagent/cell maintenance, and key technique sensitive steps. Here we discuss these critical parameters and provide a detailed protocol to encourage adoption of this technology.

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Mouse Embryonic Stem CellsOP9/OP9-DL1 Co-culture SystemHematopoietic ProgenitorsHematopoiesisT Lymphocyte DifferentiationIn Vitro DifferentiationCell Culture MaintenanceProtocolTechnique Sensitive Steps

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Cite this Article

Kučerová-Levisohn, M., Lovett, J., Lahiji, A., Holmes, R., Zúñiga-Pflücker, J. C., Ortiz, B. D. Derivation of T Cells In Vitro from Mouse Embryonic Stem Cells. J. Vis. Exp. (92), e52119, doi:10.3791/52119 (2014).

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