Detailed instructions are provided on how to train rats to voluntarily dive underwater through a 5 m long Plexiglas maze. Because the brains of rats have been very well characterized, voluntarily diving rats may help elucidate the central pathways of the mammalian diving response.
McCulloch, P. F. Training Rats to Voluntarily Dive Underwater: Investigations of the Mammalian Diving Response. J. Vis. Exp. (93), e52093, doi:10.3791/52093 (2014).