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Определение концентрации вируса через бляшки Анализы: используя традиционные и новейшие системы Наложение
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Viral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems

Определение концентрации вируса через бляшки Анализы: используя традиционные и новейшие системы Наложение

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09:28 min

November 04, 2014

DOI:

09:28 min
November 04, 2014

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Summary

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Plaque assays are the gold standard for viral quantification, utilizing entrapping overlays on host cellular monolayers to determine viral titers. While various semisolid overlays have traditionally been used, here we demonstrate plaque techniques comparing semisolid overlays to a novel liquid microcrystalline cellulose among several families of viruses.

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