JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
एक गाइड<em> Vivo</emOptogenetically पहचान Cortical निरोधात्मक interneurons से> एकल इकाई रिकॉर्डिंग
18,888 Views
•
10:32 min
•
November 07, 2014
DOI:
Summary
Automatically generated
Here we describe our strategy for obtaining stable, well-isolated single-unit recordings from identified inhibitory interneurons in the anesthetized mouse cortex. Neurons expressing ChR2 are identified by their response to blue light. The method uses standard extracellular recording equipment, and serves as an inexpensive alternative to calcium imaging or visually-guided patching.
Read Article
Cite this Article
Moore, A. K., Wehr, M. A Guide to In vivo Single-unit Recording from Optogenetically Identified Cortical Inhibitory Interneurons. J. Vis. Exp. (93), e51757, doi:10.3791/51757 (2014).
Copy