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12:43 min
July 29, 2014
DOI:
10.3791/51415-v
先天防御病毒感染是由模式识别受体(模式识别受体)触发。这两个细胞质模式识别受体RIG-I和PKR结合到病毒签名的RNA,构象变化,低聚,并激活抗病毒信号。方法被描述,其允许方便地监控构象交换和这些细胞质的PRR的低聚。
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Weber, M., Weber, F. Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE. J. Vis. Exp. (89), e51415, doi:10.3791/51415 (2014).
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