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Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins
Chapters
- 00:05Title
- 02:03Preparation of Hippocampal Slices
- 03:13Baseline Recording and LTP Induction
- 05:17Isolation of Nuclear Enriched Fractions from Hippocampal CA1
- 07:29Semiquantitative Immunoblotting for Synapto-nuclear Proteins
- 08:02Results: Induction of CA1 LTP Leads to the Accumulation of pJacS180 in the Nucleus
- 08:53Conclusion
We provide a detailed protocol for induction of long-term potentiation in the CA1 region of the hippocampus and the subsequent isolation of nuclear enriched fractions from the tetanized area of the slice. This approach can be used to determine activity dependent nuclear protein import in cellular models of learning and memory.
