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评估小鼠浆细胞树突状细胞在淋巴集结的开发利用造血祖细胞的过继转移
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JoVE 신문 면역학 및 감염병학
Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer’s Patches Using Adoptive Transfer of Hematopoietic Progenitors
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评估小鼠浆细胞树突状细胞在淋巴集结的开发利用造血祖细胞的过继转移

DOI:
10.3791/51189-v

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13:34 min

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March 17, 2014

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,
1Department of Immunology,The University of Texas MD Anderson Cancer Center, 2The University of Texas Graduate School of Biomedical Sciences

Chapters

  • 00:05Title
  • 01:56Hydrodynamic Gene Transfer
  • 02:54Adoptive Transfer of Common Dendritic Progenitors (CDPs)
  • 06:38Isolation and Enumeration of Peyer’s Patch Plasmacytoid Dendritic Cells
  • 09:36Results: Analysis of PP pDC in Ifnar/- Mice Upon Flt3L HGT and Adoptive Transfer of CPDs
  • 13:12Conclusion

Summary

자동 번역

자동 번역

这个协议描述的实验程序来评估浆细胞样树突细胞的Peyer氏从共同的树突状细胞前体细胞的补丁的分化，使用涉及FACS介导的细胞分离，水动力的基因转移，并在淋巴集结免疫子集流分析技术。

Tags

Murine Plasmacytoid Dendritic CellsPeyer’s PatchesAdoptive TransferHematopoietic ProgenitorsCommon Dendritic Cell ProgenitorsFlt3 LigandIfnar-/- MiceFlow Cytometry

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