Generation of lymph node/fat pad chimeras for the study of lymph node stromal cell origin is described. The method involves the isolation of lymph nodes from newborn mice and embryonic fat pads, the generation of chimeric lymph node-fat pads, and their transfer under the kidney capsule of a host mouse.
Benezech, C., Caamano, J. H. Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin. J. Vis. Exp. (82), e50952, doi:10.3791/50952 (2013).