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Identification of Specific Sensory Neuron Populations for Study of Expressed Ion Channels
Chapters
- 00:05Title
- 01:35Animal Preparation and Labeling
- 02:27Dissection and Neuronal Isolation
- 07:06Patch-clamp Recording
- 08:09Immunocytochemistry
- 09:42Results: Identification of Specific Sensory Neuron Populations and Patch-clamp Recording
- 11:01Conclusion
Afferent sensory neurons signal sensory information from the periphery to the central nervous system. Identifying specific afferent neurons will help in understanding their physiology. We describe a method of retrograde labeling to identify afferent neurons, and study the voltage-gated ion channels in these neurons using patch clamp electrophysiology and immunocytochemistry.
