Journal
/
면역학 및 감염병학
/
A Parasite Rescue and Transformation Assay auf leishmanientötende Screening gegen intrazelluläre<em> Leishmania donovani</em> Amastigoten in THP1 Menschliche Akute Monozytenleukämie Cell Line
JoVE 신문
면역학 및 감염병학
This content is Free Access.
JoVE 신문 면역학 및 감염병학
A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line
Please note that all translations are automatically generated. Click here for the English version.

A Parasite Rescue and Transformation Assay auf leishmanientötende Screening gegen intrazelluläre<em> Leishmania donovani</em> Amastigoten in THP1 Menschliche Akute Monozytenleukämie Cell Line

21,883 Views

•

12:22 min

•

December 30, 2012

DOI:

10.3791/4054-v

DOI:
10.3791/4054-v

•

12:22 min
December 30, 2012

•

7 Views
, , ,
1National Center for Natural Products Research, School of Pharmacy,University of Mississippi, 2Department of Pharmacology,University of Mississippi

Summary

Automatically generated

Ein Parasit-Rettungs-und Transformations-Assay mit THP1 Zellen infiziert<em> In vitro</em> Mit<em> Leishmania donovani</em> Hat für anti-Leishmania Wirkstoff-Screening optimiert. Der Test beinhaltet Differenzierung THP1 Zellen, Infektion mit Promastigoten Behandlung mit Testarzneimitteln, kontrollierte Lyse der infizierten Makrophagen, Rettung Amastigoten, Umwandlung in Promastigoten und Überwachung promastigote Wachstum und die Vermehrung mit einem fluorimetrischen Assay.

Explore More Videos

Parasite RescueTransformation AssayAntileishmanial ScreeningIntracellular Leishmania Donovani AmastigotesTHP1 Human Acute Monocytic Leukemia Cell LineLeishmaniasisNeglected DiseasesDeveloping CountriesRisk Of Contracting LeishmaniasisVisceral LeishmaniasisLimited Drug OptionsTherapeutic Doses ToxicityMultiple Drug ResistanceAnti-leishmanial Drug Discovery PipelineIn Vitro Screening ModelsPromastigotes AssayAxenic Amastigotes AssayMacrophage-amastigotes Model

Read Article

Cite this Article

Jain, S. K., Sahu, R., Walker, L. A., Tekwani, B. L. A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line. J. Vis. Exp. (70), e4054, doi:10.3791/4054 (2012).

Download .ris file

Copy

Share Video

.

Copy