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Antileishmanial筛选对细胞内的寄生虫救援和转化实验<em>杜氏利什曼原虫</em无鞭毛体在THP1人急性单核细胞白血病细胞株
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면역학 및 감염병학
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A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line
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Antileishmanial筛选对细胞内的寄生虫救援和转化实验<em>杜氏利什曼原虫</em无鞭毛体在THP1人急性单核细胞白血病细胞株

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12:22 min

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December 30, 2012

DOI:

10.3791/4054-v

DOI:
10.3791/4054-v

•

12:22 min
December 30, 2012

•

7 Views
, , ,
1National Center for Natural Products Research, School of Pharmacy,University of Mississippi, 2Department of Pharmacology,University of Mississippi

Summary

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THP1细胞感染寄生虫救援和转化试验<em在体外</em>与<em>杜氏利什曼原虫</em进行了优化为反leishmanial药物的筛选。该法涉及的THP1细胞，前鞭毛体感染，治疗与试验药物，控制受感染的巨噬细胞的溶解，抢救无鞭毛体，前鞭毛体的改造和监控前鞭毛虫的生长和增殖与荧光检测的分化。

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Parasite RescueTransformation AssayAntileishmanial ScreeningIntracellular Leishmania Donovani AmastigotesTHP1 Human Acute Monocytic Leukemia Cell LineLeishmaniasisNeglected DiseasesDeveloping CountriesRisk Of Contracting LeishmaniasisVisceral LeishmaniasisLimited Drug OptionsTherapeutic Doses ToxicityMultiple Drug ResistanceAnti-leishmanial Drug Discovery PipelineIn Vitro Screening ModelsPromastigotes AssayAxenic Amastigotes AssayMacrophage-amastigotes Model

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Cite this Article

Jain, S. K., Sahu, R., Walker, L. A., Tekwani, B. L. A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line. J. Vis. Exp. (70), e4054, doi:10.3791/4054 (2012).

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