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培養神経細胞における膜タンパク質の側方拡散とエキソサイトーシスは、蛍光回復と蛍光損失光退色を用いて評価
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February 29, 2012
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Lateral DiffusionExocytosisMembrane ProteinsCultured NeuronsFluorescence RecoveryFluorescence-loss PhotobleachingTraffickingReceptorsIon ChannelsSynaptic ProteinsSuper-ecliptic PHluorinLive Cell ImagingPlasma Membrane ProteinsProtonationPhoton AbsorptionFluorescence EmissionIntracellular Trafficking EventsLow PH CompartmentsNeutral PH Extracellular EnvironmentPhotodamagePhotobleachingFRAP (fluorescence Recovery After Photobleaching)Protein Dynamics
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Hildick, K. L., González-González, I. M., Jaskolski, F., Henley, J. M. Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching. J. Vis. Exp. (60), e3747, doi:10.3791/3747 (2012).
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