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横向扩散和培养的神经元膜蛋白的胞吐评估使用荧光恢复和荧光损失漂白
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February 29, 2012
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Lateral DiffusionExocytosisMembrane ProteinsCultured NeuronsFluorescence RecoveryFluorescence-loss PhotobleachingTraffickingReceptorsIon ChannelsSynaptic ProteinsSuper-ecliptic PHluorinLive Cell ImagingPlasma Membrane ProteinsProtonationPhoton AbsorptionFluorescence EmissionIntracellular Trafficking EventsLow PH CompartmentsNeutral PH Extracellular EnvironmentPhotodamagePhotobleachingFRAP (fluorescence Recovery After Photobleaching)Protein Dynamics
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Hildick, K. L., González-González, I. M., Jaskolski, F., Henley, J. M. Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching. J. Vis. Exp. (60), e3747, doi:10.3791/3747 (2012).
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