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横向扩散和培养的神经元膜蛋白的胞吐评估使用荧光恢复和荧光损失漂白
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February 29, 2012
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这份报告介绍了利用活细胞成像和光漂白技术，以确定表面的表达，运输途径和贩运外生表示，pH敏感的绿色荧光蛋白标记的蛋白质在神经元细胞膜动力学。
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Lateral DiffusionExocytosisMembrane ProteinsCultured NeuronsFluorescence RecoveryFluorescence-loss PhotobleachingTraffickingReceptorsIon ChannelsSynaptic ProteinsSuper-ecliptic PHluorinLive Cell ImagingPlasma Membrane ProteinsProtonationPhoton AbsorptionFluorescence EmissionIntracellular Trafficking EventsLow PH CompartmentsNeutral PH Extracellular EnvironmentPhotodamagePhotobleachingFRAP (fluorescence Recovery After Photobleaching)Protein Dynamics
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Hildick, K. L., González-González, I. M., Jaskolski, F., Henley, J. M. Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching. J. Vis. Exp. (60), e3747, doi:10.3791/3747 (2012).
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