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Determinazione ottimale attività citotossica di specifiche cellule T umane Her2neu CD8 confrontando il Cr51 saggio di rilascio al Sistema xCELLigence
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Determinazione ottimale attività citotossica di specifiche cellule T umane Her2neu CD8 confrontando il Cr51 saggio di rilascio al Sistema xCELLigence
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August 08, 2012
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Cytotoxic ActivityCD8 T CellsCr51 Release AssayXCELLigence SystemT Cell Receptor (TCR)Antigen-specific CD8 T CellsELIspotFlow CytometryCytotoxicity AssayChromium Release Assay (CRA)Gamma RadiationReproducibilityCaspase ReleaseBLT Esterase ActivityCD107 ExpressionImpedance-based AssayRoche XCelligence System
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Erskine, C. L., Henle, A. M., Knutson, K. L. Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System. J. Vis. Exp. (66), e3683, doi:10.3791/3683 (2012).
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