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Tissue Vorbereitung und Immunfärbung von Maus sensorischen Nervenfasern innervieren Haut und Limb Bones
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Tissue Preparation and Immunostaining of Mouse Sensory Nerve Fibers Innervating Skin and Limb Bones
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Tissue Vorbereitung und Immunfärbung von Maus sensorischen Nervenfasern innervieren Haut und Limb Bones

22,897 Views

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10:25 min

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January 26, 2012

DOI:

10.3791/3485-v

DOI:
10.3791/3485-v

•

10:25 min
January 26, 2012

•

5 Views
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1Department of Pharmacology,The University of Iowa, 2Department of Anesthesia,Roy J. and Lucille A. Carver College of Medicine, The University of Iowa

Summary

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Immunzytochemische Identifizierung der peripheren sensorischen Nervenfasern Subtypen (und Detektion von Protein-Expression darin) sind der Schlüssel zum Verständnis der molekularen Mechanismen, die periphere Empfindung. Hier beschreiben wir Methoden für die Herstellung von peripheren / viszeralen Gewebeproben, wie Haut-und Gliederschmerzen Knochen, für spezifische Immunfärbung der peripheren sensorischen Nervenfasern.

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Shepherd, A. J., Mohapatra, D. P. Tissue Preparation and Immunostaining of Mouse Sensory Nerve Fibers Innervating Skin and Limb Bones. J. Vis. Exp. (59), e3485, doi:10.3791/3485 (2012).

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