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RNA的分离绿脓杆菌殖民小鼠胃肠道
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract
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RNA的分离绿脓杆菌殖民小鼠胃肠道

18,782 Views

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08:52 min

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September 28, 2011

DOI:

10.3791/3293-v

DOI:
10.3791/3293-v

•

08:52 min
September 28, 2011

•

6 Views
, , ,
1Department of Pediatrics,University of Texas Southwestern Medical Center , 2Channing Laboratory, Department of Medicine, Brigham and Women’s Hospital,Harvard Medical School, 3Department of Pediatrics and Microbiology,University of Texas Southwestern Medical Center

Summary

Automatically generated

RNA提取的一种可靠的方法<em>绿脓杆菌</em从小鼠cecums恢复描述。回收的RNA是足够的数量和质量，为后续的qPCR，转录分析和RNA序列的实验。这种技术可以适用于RNA等肠道微生物的隔离。

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Lopez-Medina, E., Neubauer, M. M., Pier, G. B., Koh, A. Y. RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract. J. Vis. Exp. (55), e3293, doi:10.3791/3293 (2011).

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